Adhesion of clinical and environmental Aeromonas isolates to HEp-2 cells

Antibiotic resistance in clinical and environmental isolates of Aeromonas spp.

Role of Aeromonas hydrophila Flagella Glycosylation in Adhesion to Hep-2 Cells, Biofilm Formation and Immune Stimulation

Polar flagellin proteins from Aeromonas hydrophila strain AH-3 (serotype O34) were found to be O-glycosylated with a heterogeneous heptasaccharide glycan. Two mutants with altered (light and strong) polar flagella glycosylation still able to produce flagella were previously obtained, as well as mutants lacking the O34-antigen lipopolysaccharide (LPS) but with unaltered polar flagella glycosylat...

M protein mediates streptococcal adhesion to HEp-2 cells.

Streptococcus pyogenes adheres to human epithelial cells in vitro and in vivo. To identify adhesins, cell wall components were extracted from S. pyogenes M6 with alkali or by treatment with mutanolysin and lysozyme. HEp-2 cells were incubated with extracts of S. pyogenes M6 and then analyzed by Western blot (immunoblot) assays, using antibodies to S. pyogenes. Only one streptococcal component (...

association between efficient adhesion to hep-2 cells and severity of gastritis in helicobacter pylori strains isolated from children

background and objectives: helicobacter pylori causes chronic gastritis, peptic ulceration, and is a risk factor for gastric cancer. more than half of the world`s population is infected with this pathogen, however the disease outcome varies. among the virulence factors of h. pylori, specific adhesions to gastric epithelium may be an important step in the induction of active inflammation. h. pyl...

Relationship between adhesion to intestinal Caco-2 cells and multidrug resistance in Klebsiella pneumoniae clinical isolates.

Klebsiella pneumoniae is an opportunistic gram-negative pathogen involved in outbreaks of nosocomial infections in intensive care units. Strains are resistant to multiple antibiotics, and 15 to 30% of them are also resistant to the broad-spectrum cephalosporins by the production of R plasmid-encoded extended-spectrum beta-lactamases. Because the gastrointestinal tracts of patients have been sho...